Total RNA extraction from fish fin ray

Fishes (zebrafish or medaka) were sacrificed with an overdose of Tricaine solution (4 g/L in water).

The fishes’ caudal fin rays were cut by a scalpel.

Wash fin rays in tap water and stored in ice. 

Carefully wipe off the water on fin rays with a clean paper towel. 

Transfer fin rays into 1.5 ml tube and add 500ul Qiazol lysis reagent (Qiagen, Hilden, Germany).

Homogenized fin rays using a BioMasher homogenizer (BioMasher II, Nippi, Tokyo, Japan) for 30 sec.

BioMasher homogenizer (BioMasher II, Nippi, Tokyo, Japan)

Add 400 ml Qiazol lysis reagent to make the total volume about 900ml.

RNA extraction was performed with RNeasy Mini RNA isolation kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol.