Lipid content measurement

Cellular lipid content was measured using BODIPY 493/503. The collected cells were washed with PBS three times, and then the washed cells were stained with BODIPY493/503 contained PBS solution (1 uL dye in 100 uL PBS for 0.5-1M cells) for 15 minutes at room temperature and examined immediately using both confocal microscopy and flow cytometry. Pleaes pay more attention, during this process, any FBS or protein existed will lead to failur of this experiment. So FBS or BSA-free PBS was used to stain. 

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