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2.11. Glucose uptake and lactate production

HW Hui Wang
XL Xiaoxia Liu

Last updated date: Jun 9, 2022 Views: 553 Forks: 0

original An abbreviated version of this protocol was published in Molecular Oncology in Feb, 2021
Enhancing cancer‐associated fibroblast fatty acid catabolism within a metabolically challenging tumor microenvironment drives colon cancer peritoneal metastasis
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Indicated cells in the exponential growth phase were seeded in triplicate at a density of 2.5 × 104 cells/mL in 24-well plates. Overnight,  the medium was replaced with complete DMEM supplemented with 1% pen/strep, and the culture medium was collected after a 24-h incubation step to analyze glucose and lactate levels using a YSI 2950 STAT Plus instrument. Glucose uptake and lactate production were determined by calculating the differences in glucose and lactate concentrations between the cell culture medium and fresh medium without cells. 
 

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Wang, H and Liu, X(2022). 2.11. Glucose uptake and lactate production. Bio-protocol Preprint. bio-protocol.org/prep1710.
  2. Peng, S., Chen, D., Cai, J., Yuan, Z., Huang, B., Li, Y., Wang, H., Luo, Q., Kuang, Y., Liang, W., Liu, Z., Wang, Q., Cui, Y., Wang, H. and Liu, X.(2021). Enhancing cancer‐associated fibroblast fatty acid catabolism within a metabolically challenging tumor microenvironment drives colon cancer peritoneal metastasis. Molecular Oncology 15(5). DOI: 10.1002/1878-0261.12917

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