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Preprint

Study design

CS Christian Schwartz
PF Padraic G. Fallon

Last updated date: May 10, 2022 Views: 579 Forks: 0

original An abbreviated version of this protocol was published in Science Translational Medicine
Innate PD-L1 limits T cell–mediated adipose tissue inflammation and ameliorates diet-induced obesity
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Reaction conditions:

 

Temp.Time 
50°C 2:00 min 
95°C2:00 min 
95°C0:15 min40 cycles
60°C1:00 min
melting curve 

Related files

pdf Table1_primers.pdf download
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Schwartz, C and Fallon, P(2022). Study design. Bio-protocol Preprint. bio-protocol.org/prep1668.
  2. Schwartz, C., Schmidt, V., Deinzer, A., Hawerkamp, H. C., Hams, E., Bayerlein, J., Röger, O., Bailer, M., Krautz, C., El Gendy, A., Elshafei, M., Heneghan, H. M., Hogan, A. E., O’Shea, D. and Fallon, P. G.(2022). Innate PD-L1 limits T cell–mediated adipose tissue inflammation and ameliorates diet-induced obesity. Science Translational Medicine 14(635). DOI: 10.1126/scitranslmed.abj6879

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