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Preprint

Data Analysis

TZ Tianying Zhong
YG Yun Gu

Last updated date: Mar 27, 2022 Views: 582 Forks: 0

original An abbreviated version of this protocol was published in Technology in Cancer Research & Treatment in Feb, 2021
Label-Free Quantitative Comparison of Cervical Mucus Peptides in Subjects With Endocervical Adenocarcinoma and Adenocarcinoma in Situ
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Analysis procedure:       

1.The resulting Raw data file was analyzed with ProteinPilot. Then proteins and peptides were identified.

 

2.The identification of each sample and the total protein or peptide are screened according to unused score ≥ 1.3, confidence ≥ 95.

 

3.The chromatographic peaks are extracted from the spectrum for peptide quantification using Skyline software.

 

4.Proteins are quantified based on unique peptide’s abundance.

 

5.The significantly different proteins or peptides are detected according to |FC|≥1.5 and P-value≤0.05. Finally, significant differences in each comparison group can be obtained.

 

Copyright: Content may be subjected to copyright.
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Zhong, T and Gu, Y(2022). Data Analysis. Bio-protocol Preprint. bio-protocol.org/prep1600.
  2. Shi, F., Li, X., Ma, Z., Luan, T., Zhong, T. and Gu, Y.(2021). Label-Free Quantitative Comparison of Cervical Mucus Peptides in Subjects With Endocervical Adenocarcinoma and Adenocarcinoma in Situ. Technology in Cancer Research & Treatment 0(0). DOI: 10.1177/1533033821997825

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