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Preprint

CRISPR knockout and siRNA knockdown

ZW Zhiping Weng
KS Kevin Struhl

Last updated date: Mar 22, 2022 DOI: 10.21769/p1593 Views: 700 Forks: 0

original An abbreviated version of this protocol was published in eLife in Aug, 2021
YAP and TAZ are transcriptional co-activators of AP-1 proteins and STAT3 during breast cellular transformation
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Here is the information about TEAD siRNAs, including TEAD1, 2, 3, 4 genes. We originally ordered them from Dharmacon and now named Horizon Discover.  (https://horizondiscovery.com/)

Genes      Horizon Discover ( Original Dharmacon) Cat#
TEAD1     E-012603-00-0005
TEAD2     E-012611-00-0005
TEAD3     E-012604-00-0005
TEAD4     E-019570-00-0005

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Weng, Z and Struhl, K(2022). CRISPR knockout and siRNA knockdown. Bio-protocol Preprint. DOI: 10.21769/p1593.
  2. He, L., Pratt, H., Gao, M., Wei, F., Weng, Z. and Struhl, K.(2021). YAP and TAZ are transcriptional co-activators of AP-1 proteins and STAT3 during breast cellular transformation. eLife. DOI: 10.7554/eLife.67312

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1 Q&A

Could you post your detailed procedure of siRNA knockdown and Crisper knockdown here?
edit 0 Answer 6 Views Aug 14, 2023
This protocol preprint was submitted via the "Request a Protocol" track.