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Preprint

Preparation for two-photon calcium imaging in visual cortex

CS Carsen Stringer
MP Marius Pachitariu
KH Kenneth D. Harris

Last updated date: Mar 18, 2022 Views: 606 Forks: 0

original An abbreviated version of this protocol was published in Science in Apr, 2019
Spontaneous behaviors drive multidimensional, brainwide activity
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Hi! We used an "off-the-shelf" microscope from Thorlabs (Bergamo II) and the cranial window protocol is adapted from this detailed protocol: https://www.nature.com/articles/nprot.2014.165.

 

To save costs on the microscope and reduce its complexity, I recommend a fiber laser (however it will lock you to a specific wavelength, e.g. 920nm for gcamp). There are also cheaper microscopes available, e.g. from Neurolabware. Good luck! 

 



 

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Stringer, C, Pachitariu, M and Harris, K(2022). Preparation for two-photon calcium imaging in visual cortex. Bio-protocol Preprint. bio-protocol.org/prep1589.
  2. Stringer, C., Pachitariu, M., Steinmetz, N., Reddy, C. B., Carini, M. and Harris, K. D.(2019). Spontaneous behaviors drive multidimensional, brainwide activity. Science 364(6437). DOI: 10.1126/science.aav7893

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Biophysics

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