- Home
- Protocols
-
DNAgel synthesis
Last updated date: Dec 1, 2021 Views: 678 Forks: 0
Download PDF
Ask a
question
How to
cite
Favorite
Dissolve 20 mg deoxyribonucleic acid sodium salt (smDNA, Aldrich) in filter-sterilized (0.22 µm filter) 4.0 mM NaBr solution to get 10 wt% precursor at room temperature. The smDNA may take 24 hours to be fullly dissolved.
Carefully mix 2.5 wt% poly(ethylene glycol) diglycidyl ether (PEGDE, Mn = 500, Aldrich) with the precursor.
Carefully mix 0.5 wt% N,N,N′,N′-Tetramethylethylenediamine (TMEDA, Aldrich) with the precursor.
Centrifuge the precursor to remove extra air bubbles.
Take appropriate amount of precursor by pipette tip and apply it on target interface.
Put the functionalized interface and a sterilized gauze soaked with DI water in a plastic petri dish.
Carefully seal the petri dish by parafilm.
Put the petri dish in a chamber with controlled temperature (27 °C) and humidity (90%).
Wait for 48 hours and take out the functionalized interface.
Use DI water to rinse the DNA hydrogel to remove unreacted chemicals.
- Xiong, Z and Ho, J(2021). DNAgel synthesis. Bio-protocol Preprint. bio-protocol.org/prep1459.
- Xiong, Z., Achavananthadith, S., Lian, S., Madden, L. E., Ong, Z. X., Chua, W., Kalidasan, V., Li, Z., Liu, Z., Singh, P., Yang, H., Heussler, S. P., Kalaiselvi, S. M. P., Breese, M. B. H., Yao, H., Gao, Y., Sanmugam, K., Tee, B. C. K., Chen, P., Loke, W., Lim, C. T., Chiang, G. S. H., Tan, B. Y., Li, H., Becker, D. L. and Ho, J. S.(2021). A wireless and battery-free wound infection sensor based on DNA hydrogel. Science Advances 7(47). DOI: 10.1126/sciadv.abj1617
Category
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.
Post a Question
