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Lineage tracing method

CC Cathryn R Cadwell

Last updated date: Nov 29, 2021 Views: 734 Forks: 0

original An abbreviated version of this protocol was published in eLife in Mar, 2020
Cell type composition and circuit organization of clonally related excitatory neurons in the juvenile mouse neocortex
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Hello,

 

Thanks you for the question and we apologize if this was not clear in our methods section. We performed confocal imaging and analysis of clones at postnatal day 10, following tamoxifen administration at embryonic day 9.5, 10.5, or 11.5. Immunohistochemistry for cell type markers was performed at embryonic day 12.5.All of the multipatching and Patch-seq experiments were done at postnatal day 15-19. 

 

Best regards,

 

Cathryn Cadwell

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Cadwell, C(2021). Lineage tracing method. Bio-protocol Preprint. bio-protocol.org/prep1456.
  2. Cadwell, C. R., Scala, F., Fahey, P. G., Kobak, D., Mulherkar, S., Sinz, F. H., Papadopoulos, S., Tan, Z. H., Johnsson, P., Hartmanis, L., Li, S., Cotton, R. J., Tolias, K. F., Sandberg, R., Berens, P., Jiang, X. and Tolias, A. S.(2020). Cell type composition and circuit organization of clonally related excitatory neurons in the juvenile mouse neocortex. eLife. DOI: 10.7554/eLife.52951

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