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General experimental procedures

SB Steffen Breinlinger
TN Timo H. J. Niedermeyer
SW Susan B. Wilde

Last updated date: Oct 31, 2021 Views: 660 Forks: 0

original An abbreviated version of this protocol was published in Science in Mar, 2021
Hunting the eagle killer: A cyanobacterial neurotoxin causes vacuolar myelinopathy
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https://utex.org/products/bg-11-medium?variant=30991786868826

 

This is our standart protocol for preparation of BG11 media. For cleaner strains of cyanobacteria with heterocytes, use more likely BG11 without nitrogen. Just skip the 1st step, NaNO3.

 

Lenka Štenclová, help requested by Susan Wilde 

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Breinlinger, S, Niedermeyer, T and Wilde, S(2021). General experimental procedures. Bio-protocol Preprint. bio-protocol.org/prep1418.
  2. Breinlinger, S., Phillips, T. J., Haram, B. N., Mareš, J., Yerena, J. A. M., Hrouzek, P., Sobotka, R., Henderson, W. M., Schmieder, P., Williams, S. M., Lauderdale, J. D., Wilde, H. D., Gerrin, W., Kust, A., Washington, J. W., Wagner, C., Geier, B., Liebeke, M., Enke, H., Niedermeyer, T. H. J. and Wilde, S. B.(2021). Hunting the eagle killer: A cyanobacterial neurotoxin causes vacuolar myelinopathy. Science 371(6536). DOI: 10.1126/science.aax9050

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