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Preprint

ATAC-seq

HJ Heinrich Jasper
HT Helen Marie Tauc

Last updated date: May 6, 2021 Views: 910 Forks: 0

original An abbreviated version of this protocol was published in eLife in Mar, 2021
Age-related changes in polycomb gene regulation disrupt lineage fidelity in intestinal stem cells
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To obtain 100k intestinal stem cells, I dissected about 120 flies. Depending on the state of your flies this may vary. 

For the ATAC protocol, I followed the steps in Buenrostro et al., 2015, which describes the protocol in great detail (doi: 10.1002/0471142727.mb2129s109).

I hope this helps!

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Jasper, H and Tauc, H M(2021). ATAC-seq. Bio-protocol Preprint. bio-protocol.org/prep1059.
  2. Tauc, H. M., Rodriguez-Fernandez, I. A., Hackney, J. A., Pawlak, M., Ronnen Oron, T., Korzelius, J., Moussa, H. F., Chaudhuri, S., Modrusan, Z., Edgar, B. A. and Jasper, H.(2021). Age-related changes in polycomb gene regulation disrupt lineage fidelity in intestinal stem cells. eLife. DOI: 10.7554/eLife.62250

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