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Aptamers live staining part II.
DOI: 10.21769/BioProtoc.v94 Published: Sep 5, 2017 Views: 4505
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Video legend
This video shows removal of coverslips containing cells from the 12-well plates using half-curved-forceps and incubation of cells (on coverslips) with staining solution. Please note that this video is for demonstration only and that this step should be performed inside the cell culture hood. To avoid contamination, gloves should be sprayed with 70% ethanol.
Extracted from protocol
Staining of Membrane Receptors with Fluorescently-labeled DNA Aptamers for Super-resolution Imaging
Gomes de Castro, M. A., Höbartner, C. and Opazo, F. (2017). Bio-protocol 7(17): e2541.
DOI: 10.21769/BioProtoc.2541.
This video shows removal of coverslips containing cells from the 12-well plates using half-curved-forceps and incubation of cells (on coverslips) with staining solution. Please note that this video is for demonstration only and that this step should be performed inside the cell culture hood. To avoid contamination, gloves should be sprayed with 70% ethanol.
Extracted from protocol
Staining of Membrane Receptors with Fluorescently-labeled DNA Aptamers for Super-resolution Imaging
Gomes de Castro, M. A., Höbartner, C. and Opazo, F. (2017). Bio-protocol 7(17): e2541.
DOI: 10.21769/BioProtoc.2541.
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Keywords
Microscopy, Super-resolution, STED, Aptamers, Affinity probes
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