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EV isolation demonstration.
DOI: 10.21769/BioProtoc.v89 Published: Sep 5, 2017 Views: 10988
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This video demonstrates how to prepare the syringe and conical tube apparatus shown in Figure 3, and the procedures for extracting apoplastic wash fluid from leaves and subsequent purification of EVs from this fluid.
Extracted from protocol
Isolation and Quantification of Plant Extracellular Vesicles
Rutter, B. D., Rutter, K. L. and Innes, R. W. (2017). Bio-protocol 7(17): e2533.
DOI: 10.21769/BioProtoc.2533.
This video demonstrates how to prepare the syringe and conical tube apparatus shown in Figure 3, and the procedures for extracting apoplastic wash fluid from leaves and subsequent purification of EVs from this fluid.
Extracted from protocol
Isolation and Quantification of Plant Extracellular Vesicles
Rutter, B. D., Rutter, K. L. and Innes, R. W. (2017). Bio-protocol 7(17): e2533.
DOI: 10.21769/BioProtoc.2533.
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Keywords
Arabidopsis thaliana, Extracellular vesicles, EVs, Apoplastic wash, DiOC6, Fluorometric quantification
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