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Corneoscleral tissue processing for Sphere Forming Assay.
DOI: 10.21769/BioProtoc.v66 Published: Jul 20, 2017 Views: 3929
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Corneoscleral tissue is rubbed with a cotton bud on the posterior side to remove endothelial cells, scraped on the anterior side to remove epithelial cells and washed in PBS. The corneal/limbal region is excised and cut into small pieces prior to enzymatic treatment for limbal cell isolation.
Extracted from protocol
Implantation of Human Peripheral Corneal Spheres into Cadaveric Human Corneal Tissue
Mathan, J., Ismail, S., McGhee, J. and Sherwin, T. (2017). Bio-protocol 7(14): e2412.
DOI: 10.21769/BioProtoc.2412.
Corneoscleral tissue is rubbed with a cotton bud on the posterior side to remove endothelial cells, scraped on the anterior side to remove epithelial cells and washed in PBS. The corneal/limbal region is excised and cut into small pieces prior to enzymatic treatment for limbal cell isolation.
Extracted from protocol
Implantation of Human Peripheral Corneal Spheres into Cadaveric Human Corneal Tissue
Mathan, J., Ismail, S., McGhee, J. and Sherwin, T. (2017). Bio-protocol 7(14): e2412.
DOI: 10.21769/BioProtoc.2412.
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Keywords
Cell Culture, Cornea, Sphere-forming cells, Implantation, Limbus
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