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Making a nylon wool column.
DOI: 10.21769/BioProtoc.v59 Published: Jul 5, 2017 Views: 5890
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20 ml of RP10 medium are added to nylon wool and stirred until the medium uniformly spreads in the wool.
Extracted from protocol
EAE Induction by Passive Transfer of MOG-specific CD4+ T Cells
Tanaka, Y., Arima, Y., Higuchi, K., Ohki, T., Elfeky, M., Ota, M., Kamimura, D. and Murakami, M. (2017). Bio-protocol 7(13): e2370.
DOI: 10.21769/BioProtoc.2370.
20 ml of RP10 medium are added to nylon wool and stirred until the medium uniformly spreads in the wool.
Extracted from protocol
EAE Induction by Passive Transfer of MOG-specific CD4+ T Cells
Tanaka, Y., Arima, Y., Higuchi, K., Ohki, T., Elfeky, M., Ota, M., Kamimura, D. and Murakami, M. (2017). Bio-protocol 7(13): e2370.
DOI: 10.21769/BioProtoc.2370.
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Keywords
Experimental autoimmune encephalomyelitis, Pathogenic CD4+ T cells, Myelin oligodendrocyte glycoprotein, Gateway reflex, Passive transfer
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