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Visualization of the interphase formation during preparation of a step gradient.
DOI: 10.21769/BioProtoc.v560 Published: Dec 5, 2019 Views: 1595
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The tube contains 10% sucrose (transparent). We underlay with the 40% sucrose (blue) using a syringe. To better visualize the interphase formation, we stained the 40% sucrose solution here with a blue dye. If preparing gradients for virus preparations we do not add any dye. It is crucial to add the heavy solution smoothly without pressing the needle to the bottom of the tube and without pushing the plunger too abruptly. An interphase between the 10% and 40% solution forms and migrates up.
Extracted from protocol
Herpes Simplex Virus Type 1 Propagation, Titration and Single-step Growth Curves
Grosche, L., Döhner, K., Düthorn, A., Hickford-Martinez, A., Steinkasserer, A. and Sodeik, B. (2019). Bio-protocol (): e3441.
DOI: 10.21769/BioProtoc.3441.
The tube contains 10% sucrose (transparent). We underlay with the 40% sucrose (blue) using a syringe. To better visualize the interphase formation, we stained the 40% sucrose solution here with a blue dye. If preparing gradients for virus preparations we do not add any dye. It is crucial to add the heavy solution smoothly without pressing the needle to the bottom of the tube and without pushing the plunger too abruptly. An interphase between the 10% and 40% solution forms and migrates up.
Extracted from protocol
Herpes Simplex Virus Type 1 Propagation, Titration and Single-step Growth Curves
Grosche, L., Döhner, K., Düthorn, A., Hickford-Martinez, A., Steinkasserer, A. and Sodeik, B. (2019). Bio-protocol (): e3441.
DOI: 10.21769/BioProtoc.3441.
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Keywords
Herpes simplex virus type 1, Virus propagation, Plaque assay, Single-step growth curve
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