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Injection of an axon-tracer plasmid into the neural tube of a mouse embryo.
DOI: 10.21769/BioProtoc.v556 Published: Sep 20, 2019 Views: 1137
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Extracted from protocol
Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation
Kinoshita-Kawada, M., Hasegawa, H., Hongu, T., Yanagi, S., Kanaho, Y., Masai, I., Mishima, T., Chen, X., Tsuboi, Y., Rao, Y., Yuasa-Kawada, J. and Wu, J. Y. (2019). Bio-protocol 9(18): e3373.
DOI: 10.21769/BioProtoc.3373.
Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation
Kinoshita-Kawada, M., Hasegawa, H., Hongu, T., Yanagi, S., Kanaho, Y., Masai, I., Mishima, T., Chen, X., Tsuboi, Y., Rao, Y., Yuasa-Kawada, J. and Wu, J. Y. (2019). Bio-protocol 9(18): e3373.
DOI: 10.21769/BioProtoc.3373.
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Keywords
Explant culture, Spinal cord, Mouse embryos, Midline, Floor plate, Commissural axons, Axon guidance, Gene knockdown
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