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Transferring lipid extracts in the lower-phase with Pasteur pipette.
DOI: 10.21769/BioProtoc.v530 Published: Jun 20, 2019 Views: 2229
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Holding vials between left thumb and index fingers with ~65°, insert pipette tip into the bottom layer to the lowest corner of the sample vial. Slowly aspirate the lower-phase solution only (~900 µl) with pipette-aid, and then transfer it into the new glass vial.
Extracted from protocol
Fluorescence HPLC Analysis of the in-vivo Activity of Glucosylceramide Synthase
Roy, K. R., Khiste, S. K., Liu, Z. and Liu, Y. (2019). Bio-protocol 9(12): e3269.
DOI: 10.21769/BioProtoc.3269.
Holding vials between left thumb and index fingers with ~65°, insert pipette tip into the bottom layer to the lowest corner of the sample vial. Slowly aspirate the lower-phase solution only (~900 µl) with pipette-aid, and then transfer it into the new glass vial.
Extracted from protocol
Fluorescence HPLC Analysis of the in-vivo Activity of Glucosylceramide Synthase
Roy, K. R., Khiste, S. K., Liu, Z. and Liu, Y. (2019). Bio-protocol 9(12): e3269.
DOI: 10.21769/BioProtoc.3269.
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Keywords
In-vivo enzyme activity, Glucosylceramide synthase, Ceramide, High-performance liquid chromatography, Nanomicelles, Rubusoside, Fluorescence NBD, Cancer
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