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Example of testing data from FreezeFrame4 Trial Viewer.
DOI: 10.21769/BioProtoc.v512 Published: Sep 20, 2018 Views: 2504
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This video depicts 120 sec of olfactory fear testing 24 h after olfactory fear conditioning with all of the data provided from FreezeFrame4 (video playback in real time). The conditioned odor is presented at 60 sec for 20 sec and the clean air and vacuum are turned off at 60 sec for a total of 60 sec. The Animal menu on the left shows all Animal IDs recorded under the same data file, with the selected trial highlighted in blue. The Motion Index graph indicates the recorded activity (y-axis; yellow line) across time (x-axis). The vertical blue line moves in real time with the video while the horizontal blue line shows the user-defined threshold for freezing. The rectangle below shows bouts of freezing, as determined by user-defined threshold and bout, as yellow boxes. Periods of non-freezing are not colored. The next rectangle below provides a visual for the protocol and shows the onset/offset of all stimuli during the protocol (blue rectangle: odor onset, yellow rectangle: vacuum offset, green rectangles: clean air offset). The motion index histogram should be used to set threshold. The bout length can be set to fit specific needs; in this case, 2 sec. The video to the right of the histogram shows the behavior of the mouse throughout testing, with bouts of freezing (determined through the user-defined threshold and bout length) indicated by a green light above. In this example, the mouse freezes for 0% of the 1st 60 sec interval (baseline freezing) and 74.28% during the 2nd 60 sec interval during the presentation of the conditioned stimulus.
Extracted from protocol
Assessing Classical Olfactory Fear Conditioning by Behavioral Freezing in Mice
Ross, J. M. and Fletcher, M. L. (2018). Bio-protocol 8(18): e3013.
DOI: 10.21769/BioProtoc.3013.
This video depicts 120 sec of olfactory fear testing 24 h after olfactory fear conditioning with all of the data provided from FreezeFrame4 (video playback in real time). The conditioned odor is presented at 60 sec for 20 sec and the clean air and vacuum are turned off at 60 sec for a total of 60 sec. The Animal menu on the left shows all Animal IDs recorded under the same data file, with the selected trial highlighted in blue. The Motion Index graph indicates the recorded activity (y-axis; yellow line) across time (x-axis). The vertical blue line moves in real time with the video while the horizontal blue line shows the user-defined threshold for freezing. The rectangle below shows bouts of freezing, as determined by user-defined threshold and bout, as yellow boxes. Periods of non-freezing are not colored. The next rectangle below provides a visual for the protocol and shows the onset/offset of all stimuli during the protocol (blue rectangle: odor onset, yellow rectangle: vacuum offset, green rectangles: clean air offset). The motion index histogram should be used to set threshold. The bout length can be set to fit specific needs; in this case, 2 sec. The video to the right of the histogram shows the behavior of the mouse throughout testing, with bouts of freezing (determined through the user-defined threshold and bout length) indicated by a green light above. In this example, the mouse freezes for 0% of the 1st 60 sec interval (baseline freezing) and 74.28% during the 2nd 60 sec interval during the presentation of the conditioned stimulus.
Extracted from protocol
Assessing Classical Olfactory Fear Conditioning by Behavioral Freezing in Mice
Ross, J. M. and Fletcher, M. L. (2018). Bio-protocol 8(18): e3013.
DOI: 10.21769/BioProtoc.3013.
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Keywords
Classical conditioning, Olfactory conditioning, Fear learning, Behavior, Freezing, Olfactory fear
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