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Vacuum infiltration for staining of endophytes in grass.
DOI: 10.21769/BioProtoc.v510 Published: Sep 5, 2018 Views: 3545
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Seal the lid by turning the upper stopcock. Open the side stopcock connecting the chamber to the water jet pump. Turn the water on, vacuum is now applied to the chamber. Check after a few seconds that vacuum is established, you cannot slide the lid to the left or right. Wait 2 min. For ventilation of the chamber, close the side stopcock in order to avoid water being sucked into the chamber. Turn the water off. Open the upper stopcock on the lid slowly. You should hear a hissing sound when air flows into the chamber. Repeat the procedure two more times.
Extracted from protocol
Artificial Inoculation of Epichloë festucae into Lolium perenne, and Visualisation of Endophytic and Epiphyllous Fungal Growth
Becker, Y., Green, K. A., Scott, B. and Becker, M. (2018). Bio-protocol 8(17): e2990.
DOI: 10.21769/BioProtoc.2990.
Seal the lid by turning the upper stopcock. Open the side stopcock connecting the chamber to the water jet pump. Turn the water on, vacuum is now applied to the chamber. Check after a few seconds that vacuum is established, you cannot slide the lid to the left or right. Wait 2 min. For ventilation of the chamber, close the side stopcock in order to avoid water being sucked into the chamber. Turn the water off. Open the upper stopcock on the lid slowly. You should hear a hissing sound when air flows into the chamber. Repeat the procedure two more times.
Extracted from protocol
Artificial Inoculation of Epichloë festucae into Lolium perenne, and Visualisation of Endophytic and Epiphyllous Fungal Growth
Becker, Y., Green, K. A., Scott, B. and Becker, M. (2018). Bio-protocol 8(17): e2990.
DOI: 10.21769/BioProtoc.2990.
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Keywords
Expressorium, Fungal endophyte, Inoculation, Epiphyllous Growth, Epichloë, Confocal Laser Scanning Microscopy
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