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Manipulation of A. nidulans for growth and isolation of conidia.

F Fernandes et al.

DOI: 10.21769/BioProtoc.v50 Published: Jun 5, 2017 Views: 6021

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This video shows step-by-step the manipulation of A. nidulans for growth and recovery in YUU (solid media), and isolation of the conidia by filtration using a syringe containing Miracloth filter or glass wool.

Extracted from protocol
A Reliable Assay to Evaluate the Virulence of Aspergillus nidulans Using the Alternative Animal Model Galleria mellonella (Lepidoptera)
Fernandes, C. M., Fonseca, F. L., Goldman, G. H., Pereira, M. D. and Kurtenbach, E. (2017). Bio-protocol 7(11): e2329.
DOI: 10.21769/BioProtoc.2329.

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Keywords

Aspergillus nidulans, Galleria mellonella, Fungal pathogenicity, Fungal virulence, Alternative models

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