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Analysis of ex vivo follicle rupture assay

K Knapp et al.

DOI: 10.21769/BioProtoc.v474 Published: May 20, 2018 Views: 3636

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Analysis of ex vivo follicle rupture assay. Open images acquired at the end of each experiment (both bright-field and fluorescent images) in ImageJ. Count the total number of follicles using the multi-point tool in the bright-field image. In this example, there are a total of 27 follicles. Then, count the total number of ruptured follicles in the fluorescent image. In this example, there are a total of 20 ruptured follicles; therefore, 74% of follicles ruptured in this example.

Extracted from protocol
Ex vivo Follicle Rupture and in situ Zymography in Drosophila
Knapp, E. M., Deady, L. D. and Sun, J. (2018). Bio-protocol 8(10): e2846.
DOI: 10.21769/BioProtoc.2846.

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Keywords

Drosophila, Ovulation, Follicle rupture, Octopamine, in situ zymography, Follicle cells

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