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Chlamydomonas transformation demonstration
DOI: 10.21769/BioProtoc.v472 Published: May 5, 2018 Views: 5962
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Chlamydomonas transformation demonstration. This video demonstrates how to perform nuclear transformation of Chlamydomonas reinhardtii cells by electroporation and corresponds to Steps B9-B13 of the protocol. 250 µl cells are added to each sterile cuvette. 5 µl digested DNA is added to the cuvette and mixed. The mixture of cells and DNA are briefly incubated in a 16 °C water bath prior to electroporation. After electroporation, transformed cells are transferred from the cuvette to a 15 ml centrifuge tube containing 10 ml TAP 40 mM sucrose.
Extracted from protocol
Nuclear Transformation of Chlamydomonas reinhardtii by Electroporation
Wittkopp, T. M. (2018). Bio-101: e2837.
DOI: 10.21769/BioProtoc.2837.
Chlamydomonas transformation demonstration. This video demonstrates how to perform nuclear transformation of Chlamydomonas reinhardtii cells by electroporation and corresponds to Steps B9-B13 of the protocol. 250 µl cells are added to each sterile cuvette. 5 µl digested DNA is added to the cuvette and mixed. The mixture of cells and DNA are briefly incubated in a 16 °C water bath prior to electroporation. After electroporation, transformed cells are transferred from the cuvette to a 15 ml centrifuge tube containing 10 ml TAP 40 mM sucrose.
Extracted from protocol
Nuclear Transformation of Chlamydomonas reinhardtii by Electroporation
Wittkopp, T. M. (2018). Bio-101: e2837.
DOI: 10.21769/BioProtoc.2837.
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Keywords
Algae, Nuclear transformation, Electroporation, Fluorescent fusion protein, Venus, mCherry, FLAG tag, His tag, Paromomycin, Hygromycin
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