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SearchGUI/PeptideShaker set-up.
DOI: 10.21769/BioProtoc.v463 Published: Mar 20, 2018 Views: 5018
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Walkthrough of how to set up SearchGUI to identify peptides based on fragmentation data collected using MS/MS. The search results from SearchGUI will be opened in PeptideShaker. PeptideShaker provides a user friendly interface for the examination of identified peptides, which shows the protein coverage and number of peptides identified.
Extracted from protocol
Hydrogen Deuterium Exchange Mass Spectrometry of Oxygen Sensitive Proteins
Berry, L., Patterson, A., Pence, N., Peters, J. W. and Bothner, B. (2018). Bio-protocol 8(6): e2769.
DOI: 10.21769/BioProtoc.2769.
Walkthrough of how to set up SearchGUI to identify peptides based on fragmentation data collected using MS/MS. The search results from SearchGUI will be opened in PeptideShaker. PeptideShaker provides a user friendly interface for the examination of identified peptides, which shows the protein coverage and number of peptides identified.
Extracted from protocol
Hydrogen Deuterium Exchange Mass Spectrometry of Oxygen Sensitive Proteins
Berry, L., Patterson, A., Pence, N., Peters, J. W. and Bothner, B. (2018). Bio-protocol 8(6): e2769.
DOI: 10.21769/BioProtoc.2769.
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Keywords
HDX-MS, Mass spectrometry, Anaerobic proteins, H/D exchange, Protein dynamics, Protein-protein interactions, Protein-ligand interactions
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