PAWs set-up.

B Berry et al.

DOI: 10.21769/BioProtoc.v462 Published: Mar 20, 2018 Views: 5019

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Walkthrough of how to set up protein analysis worksheet (PAWs) for the identification of unique peptides. Walkthrough includes how to load a sequence, develop theoretical cleavage map, and input mass lists for peptide identification.

Extracted from protocol
Hydrogen Deuterium Exchange Mass Spectrometry of Oxygen Sensitive Proteins
Berry, L., Patterson, A., Pence, N., Peters, J. W. and Bothner, B. (2018). Bio-protocol 8(6): e2769.
DOI: 10.21769/BioProtoc.2769.

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Keywords

HDX-MS, Mass spectrometry, Anaerobic proteins, H/D exchange, Protein dynamics, Protein-protein interactions, Protein-ligand interactions

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