Operation of the cryostat.

P Petshow et al.

DOI: 10.21769/BioProtoc.v449 Published: Feb 5, 2018 Views: 4619

Log in to your account to watch the full video
Video legend
The anti-roll plate, which is lowered at the beginning of the video, ensures the section remains flat. Note tissue sections will adhere to the microscope slide after gently coming in contact.

Extracted from protocol
Preparation of Precisely Oriented Cryosections of Undistorted Drosophila Wing Imaginal Discs for High Resolution Confocal Imaging
Petshow, S. . and Wehrli, M. (2018). Bio-protocol 8(3): e2725.
DOI: 10.21769/BioProtoc.2725.

Q&A Q&A
nfavorite Favorite

Keywords

Cryosection, Frozen sections, Confocal microscopy, Wing imaginal disc, Drosophila

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A