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Operation of the cryostat.
DOI: 10.21769/BioProtoc.v449 Published: Feb 5, 2018 Views: 4635
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The anti-roll plate, which is lowered at the beginning of the video, ensures the section remains flat. Note tissue sections will adhere to the microscope slide after gently coming in contact.
Extracted from protocol
Preparation of Precisely Oriented Cryosections of Undistorted Drosophila Wing Imaginal Discs for High Resolution Confocal Imaging
Petshow, S. . and Wehrli, M. (2018). Bio-protocol 8(3): e2725.
DOI: 10.21769/BioProtoc.2725.
The anti-roll plate, which is lowered at the beginning of the video, ensures the section remains flat. Note tissue sections will adhere to the microscope slide after gently coming in contact.
Extracted from protocol
Preparation of Precisely Oriented Cryosections of Undistorted Drosophila Wing Imaginal Discs for High Resolution Confocal Imaging
Petshow, S. . and Wehrli, M. (2018). Bio-protocol 8(3): e2725.
DOI: 10.21769/BioProtoc.2725.
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Keywords
Cryosection, Frozen sections, Confocal microscopy, Wing imaginal disc, Drosophila
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