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The reference movie of the microinjection into the two-cell stage eggs.
DOI: 10.21769/BioProtoc.v447 Published: Feb 5, 2018 Views: 4024
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Video legend
Unlike the egg holder with a silicone channel described in this protocol, the egg is fitted a channel between glass plates in this movie. The first injection is performed with the one-cell stage egg, while the second injection is performed with the two-cell stage egg. It is recommended that the solution be injected into the one-cell stage eggs, because the two-cell stage eggs are required to be injected into each cytoplasm as shown in the second injection in this video. It should be noted that the microinjection into the eggs later than two-cell stage strongly reduces the efficiency of the injection, and leads to a high degree of mosaicism in the eggs. The injected solution gives a red color with a phenol red, so that can be visible in the egg in this movie, however, it is unnecessary for the practical experiment.
Extracted from protocol
Medaka-microinjection with an Upright Microscope
Murakami, Y. and Kinoshita, M. (2018). Bio-protocol 8(3): e2716.
DOI: 10.21769/BioProtoc.2716.
Unlike the egg holder with a silicone channel described in this protocol, the egg is fitted a channel between glass plates in this movie. The first injection is performed with the one-cell stage egg, while the second injection is performed with the two-cell stage egg. It is recommended that the solution be injected into the one-cell stage eggs, because the two-cell stage eggs are required to be injected into each cytoplasm as shown in the second injection in this video. It should be noted that the microinjection into the eggs later than two-cell stage strongly reduces the efficiency of the injection, and leads to a high degree of mosaicism in the eggs. The injected solution gives a red color with a phenol red, so that can be visible in the egg in this movie, however, it is unnecessary for the practical experiment.
Extracted from protocol
Medaka-microinjection with an Upright Microscope
Murakami, Y. and Kinoshita, M. (2018). Bio-protocol 8(3): e2716.
DOI: 10.21769/BioProtoc.2716.
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Keywords
Fish, Medaka, Microinjection, Upright microscope, Genome editing, Transgenesis
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