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Automatic detection and measurement of individual Z-discs.
DOI: 10.21769/BioProtoc.v443 Published: Nov 20, 2017 Views: 5793
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Screen-recorded video showing the procedure in Fiji to obtain individual Z-discs and measure some properties of the isolated discs. First, a thresholded image is made using the automatic threshold tool. Then, individual Z-disc are obtained using the ‘Analyze particles’ tool. The particle size and the circularity options may be adjusted to optimize the procedure.
Extracted from protocol
Rapid IFM Dissection for Visualizing Fluorescently Tagged Sarcomeric Proteins
Xiao, Y., Schöck, F. and González-Morales, N. (2017). Bio-protocol 7(22): e2606.
DOI: 10.21769/BioProtoc.2606.
Screen-recorded video showing the procedure in Fiji to obtain individual Z-discs and measure some properties of the isolated discs. First, a thresholded image is made using the automatic threshold tool. Then, individual Z-disc are obtained using the ‘Analyze particles’ tool. The particle size and the circularity options may be adjusted to optimize the procedure.
Extracted from protocol
Rapid IFM Dissection for Visualizing Fluorescently Tagged Sarcomeric Proteins
Xiao, Y., Schöck, F. and González-Morales, N. (2017). Bio-protocol 7(22): e2606.
DOI: 10.21769/BioProtoc.2606.
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Keywords
Dissection, Drosophila, GFP, Indirect flight muscle, Sarcomere, Z-disc
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