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Demonstration of the procedure to cut out the Transwell membrane using a razor blade.
DOI: 10.21769/BioProtoc.v434 Published: Feb 5, 2018 Views: 4887
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The lid of the multiwall dish is covered with Parafilm and used as a support surface.
Extracted from protocol
Tracking Endocytosis and Intracellular Trafficking of Epitope-tagged Syntaxin 3 by Antibody Feeding in Live, Polarized MDCK Cells
Giovannone, A. J., Reales, E., Bhattaram, P., Fraile-Ramos, A. and Weimbs, T. (2018). Bio-protocol 8(3): e2453.
DOI: 10.21769/BioProtoc.2453.
The lid of the multiwall dish is covered with Parafilm and used as a support surface.
Extracted from protocol
Tracking Endocytosis and Intracellular Trafficking of Epitope-tagged Syntaxin 3 by Antibody Feeding in Live, Polarized MDCK Cells
Giovannone, A. J., Reales, E., Bhattaram, P., Fraile-Ramos, A. and Weimbs, T. (2018). Bio-protocol 8(3): e2453.
DOI: 10.21769/BioProtoc.2453.
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Keywords
Antibody-feeding assay, Syntaxin, SNARE, Internalization, Apical-basolateral polarity, Epithelial cells, MDCK cells
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