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Surgical excision of a wedge of limbal tissue.
DOI: 10.21769/BioProtoc.v426 Published: Jul 20, 2017 Views: 4210
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Fine forceps were used with the non-dominant hand to anchor the sclera and provide counter-traction as two diagonal ~3 mm incisions were made prior to gripping the apex of the incised tissue to cut away and remove the anterior tissue segment. Cornea-limbus-sclera demarcations have to be visually approximated.
Extracted from protocol
Implantation of Human Peripheral Corneal Spheres into Cadaveric Human Corneal Tissue
Mathan, J., Ismail, S., McGhee, J. and Sherwin, T. (2017). Bio-protocol 7(14): e2412.
DOI: 10.21769/BioProtoc.2412.
Fine forceps were used with the non-dominant hand to anchor the sclera and provide counter-traction as two diagonal ~3 mm incisions were made prior to gripping the apex of the incised tissue to cut away and remove the anterior tissue segment. Cornea-limbus-sclera demarcations have to be visually approximated.
Extracted from protocol
Implantation of Human Peripheral Corneal Spheres into Cadaveric Human Corneal Tissue
Mathan, J., Ismail, S., McGhee, J. and Sherwin, T. (2017). Bio-protocol 7(14): e2412.
DOI: 10.21769/BioProtoc.2412.
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Keywords
Cell Culture, Cornea, Sphere-forming cells, Implantation, Limbus
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