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Loading C. elegans into 24-well plates for the arsenite oxidative stress assay.
DOI: 10.21769/BioProtoc.v416 Published: Jul 5, 2017 Views: 7447
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10-12 C. elegans were transferred into a well of the 24-well plate that contains a 50 μl drop of M9. Worms should be freely trashing. Exclude non-moving worms (marked in Figure 3) before filling up wells with the As solution.
Extracted from protocol
Oxidative Stress Assays (arsenite and tBHP) in Caenorhabditis elegans
Ewald, C. Y., Hourihan, J. M. and Blackwell, T. K. (2017). Bio-protocol 7(13): e2365.
DOI: 10.21769/BioProtoc.2365.
10-12 C. elegans were transferred into a well of the 24-well plate that contains a 50 μl drop of M9. Worms should be freely trashing. Exclude non-moving worms (marked in Figure 3) before filling up wells with the As solution.
Extracted from protocol
Oxidative Stress Assays (arsenite and tBHP) in Caenorhabditis elegans
Ewald, C. Y., Hourihan, J. M. and Blackwell, T. K. (2017). Bio-protocol 7(13): e2365.
DOI: 10.21769/BioProtoc.2365.
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Keywords
Hydrogen peroxide, ROS, Xenobiotics, SKN-1, DAF-16
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