Short tutorial for membrane-based 3D image segmentation using Imaris Cell.

M Mendocilla Sato et al.

DOI: 10.21769/BioProtoc.v414 Published: Jun 20, 2017 Views: 7261

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Selected points of the protocol are presented as follows: D. 3D-reconstruction and image processing: 2. Inspect the image quality, 3. Down-sampling, 5. in silico dissection, 6. Free rotate; E. Image segmentation and labelling: 1. Automated segmentation, 4. Cell labelling.

Extracted from protocol
Analysis of 3D Cellular Organization of Fixed Plant Tissues Using a User-guided Platform for Image Segmentation
Mendocilla Sato, E. and Baroux, C. (2017). Bio-protocol 7(12): e2355.
DOI: 10.21769/BioProtoc.2355.

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Keywords

High-resolution 3D imaging, Plant tissues, Image segmentation, Morphodynamics, Tissue patterning, Arabidopsis, Ovule primordium

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