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Pattern stimulation of the neuronal culture.
DOI: 10.21769/BioProtoc.v412 Published: Jun 20, 2017 Views: 7188
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36 regions of interest (ROIs) are drawn around stimulated neurons (blue squares). Each stimulated neuron is activated by a train of light pulses (the actual stimulus is shown in blue on the top left corner). Three neurons are recorded simultaneously in cell-attached mode (yellow raster plots of evoked spikes in each corner, 15 repetitions of the stimulus) and then in whole-cell configuration (yellow trace of membrane potential, average of 7 repetitions).
Extracted from protocol
Optogenetic Stimulation and Recording of Primary Cultured Neurons with Spatiotemporal Control
Barral, J. and Reyes, A. D. (2017). Bio-protocol 7(12): e2335.
DOI: 10.21769/BioProtoc.2335.
36 regions of interest (ROIs) are drawn around stimulated neurons (blue squares). Each stimulated neuron is activated by a train of light pulses (the actual stimulus is shown in blue on the top left corner). Three neurons are recorded simultaneously in cell-attached mode (yellow raster plots of evoked spikes in each corner, 15 repetitions of the stimulus) and then in whole-cell configuration (yellow trace of membrane potential, average of 7 repetitions).
Extracted from protocol
Optogenetic Stimulation and Recording of Primary Cultured Neurons with Spatiotemporal Control
Barral, J. and Reyes, A. D. (2017). Bio-protocol 7(12): e2335.
DOI: 10.21769/BioProtoc.2335.
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Keywords
Primary culture of neurons, Optogenetics, Patterned optical stimulation
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