Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Pattern stimulation of the neuronal culture.
DOI: 10.21769/BioProtoc.v412 Published: Jun 20, 2017 Views: 7075
Log in to your account to watch the full video
Video legend
36 regions of interest (ROIs) are drawn around stimulated neurons (blue squares). Each stimulated neuron is activated by a train of light pulses (the actual stimulus is shown in blue on the top left corner). Three neurons are recorded simultaneously in cell-attached mode (yellow raster plots of evoked spikes in each corner, 15 repetitions of the stimulus) and then in whole-cell configuration (yellow trace of membrane potential, average of 7 repetitions).
Extracted from protocol
Optogenetic Stimulation and Recording of Primary Cultured Neurons with Spatiotemporal Control
Barral, J. and Reyes, A. D. (2017). Bio-protocol 7(12): e2335.
DOI: 10.21769/BioProtoc.2335.
36 regions of interest (ROIs) are drawn around stimulated neurons (blue squares). Each stimulated neuron is activated by a train of light pulses (the actual stimulus is shown in blue on the top left corner). Three neurons are recorded simultaneously in cell-attached mode (yellow raster plots of evoked spikes in each corner, 15 repetitions of the stimulus) and then in whole-cell configuration (yellow trace of membrane potential, average of 7 repetitions).
Extracted from protocol
Optogenetic Stimulation and Recording of Primary Cultured Neurons with Spatiotemporal Control
Barral, J. and Reyes, A. D. (2017). Bio-protocol 7(12): e2335.
DOI: 10.21769/BioProtoc.2335.
Q&A
Favorite
Keywords
Primary culture of neurons, Optogenetics, Patterned optical stimulation
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
0/150
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.
Post a Question0 Q&A
