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Z-stack images of MDCK cells stably expressing GFP-tubulin before (pre-TUSP) and after TUSP (post-TUSP).
DOI: 10.21769/BioProtoc.v407 Published: Apr 20, 2017 Views: 5514
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Images are shown from the basolateral to apical membranes (i.e., bottom to the top). The white arrowheads point to the midbody remnants. The circle indicates the zone subjected to TUSP. Note that the remnant of the cell subjected to TUSP was completely removed.
Extracted from protocol
Physical Removal of the Midbody Remnant from Polarised Epithelial Cells Using Take-Up by Suction Pressure (TUSP)
Bernabé-Rubio, M., Gershlick, D. C. and Alonso, M. A. (2017). Bio-protocol 7(8): e2244.
DOI: 10.21769/BioProtoc.2244.
Images are shown from the basolateral to apical membranes (i.e., bottom to the top). The white arrowheads point to the midbody remnants. The circle indicates the zone subjected to TUSP. Note that the remnant of the cell subjected to TUSP was completely removed.
Extracted from protocol
Physical Removal of the Midbody Remnant from Polarised Epithelial Cells Using Take-Up by Suction Pressure (TUSP)
Bernabé-Rubio, M., Gershlick, D. C. and Alonso, M. A. (2017). Bio-protocol 7(8): e2244.
DOI: 10.21769/BioProtoc.2244.
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Keywords
Epithelial cells, Midbody remnant, Primary cilium, Suction pressure, Patch-clamp equipment
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