Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Z-stack images of MDCK cells stably expressing GFP-tubulin before TUSP.
DOI: 10.21769/BioProtoc.v405 Published: Apr 20, 2017 Views: 5557
Log in to your account to watch the full video
Video legend
Images are shown from the basolateral to apical membranes (i.e., bottom to the top). The white arrowheads point to the midbody remnants. Note that the remnants are localised at the apical cell surface.
Extracted from protocol
Physical Removal of the Midbody Remnant from Polarised Epithelial Cells Using Take-Up by Suction Pressure (TUSP)
Bernabé-Rubio, M., Gershlick, D. C. and Alonso, M. A. (2017). Bio-protocol 7(8): e2244.
DOI: 10.21769/BioProtoc.2244.
Images are shown from the basolateral to apical membranes (i.e., bottom to the top). The white arrowheads point to the midbody remnants. Note that the remnants are localised at the apical cell surface.
Extracted from protocol
Physical Removal of the Midbody Remnant from Polarised Epithelial Cells Using Take-Up by Suction Pressure (TUSP)
Bernabé-Rubio, M., Gershlick, D. C. and Alonso, M. A. (2017). Bio-protocol 7(8): e2244.
DOI: 10.21769/BioProtoc.2244.
Q&A
Favorite
Keywords
Epithelial cells, Midbody remnant, Primary cilium, Suction pressure, Patch-clamp equipment
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
0/150
+
Post a Question0 Q&A
