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Z-stack images of MDCK cells stably expressing GFP-tubulin before TUSP.
DOI: 10.21769/BioProtoc.v405 Published: Apr 20, 2017 Views: 5483
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Images are shown from the basolateral to apical membranes (i.e., bottom to the top). The white arrowheads point to the midbody remnants. Note that the remnants are localised at the apical cell surface.
Extracted from protocol
Physical Removal of the Midbody Remnant from Polarised Epithelial Cells Using Take-Up by Suction Pressure (TUSP)
Bernabé-Rubio, M., Gershlick, D. C. and Alonso, M. A. (2017). Bio-protocol 7(8): e2244.
DOI: 10.21769/BioProtoc.2244.
Images are shown from the basolateral to apical membranes (i.e., bottom to the top). The white arrowheads point to the midbody remnants. Note that the remnants are localised at the apical cell surface.
Extracted from protocol
Physical Removal of the Midbody Remnant from Polarised Epithelial Cells Using Take-Up by Suction Pressure (TUSP)
Bernabé-Rubio, M., Gershlick, D. C. and Alonso, M. A. (2017). Bio-protocol 7(8): e2244.
DOI: 10.21769/BioProtoc.2244.
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Keywords
Epithelial cells, Midbody remnant, Primary cilium, Suction pressure, Patch-clamp equipment
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