Isolation of hind limb muscles from mouse.

H Hindi et al.

DOI: 10.21769/BioProtoc.v40 Published: May 5, 2017 Views: 14706

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This video shows basic steps for isolation of hind limb muscles from euthanized mouse and transfer them into a 6-well plate containing collection/washing/mincing solution.

Extracted from protocol
Isolation, Culturing, and Differentiation of Primary Myoblasts from Skeletal Muscle of Adult Mice
Hindi, L., McMillan, J. D., Afroze, D., Hindi, S. M. and Kumar, A. (2017). Bio-protocol 7(9): e2248.
DOI: 10.21769/BioProtoc.2248.

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Keywords

Myoblast, Skeletal muscle, Myogenesis, MyoD, Pax7, Myogenin, Myogenic differentiation, Hind limb muscle

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