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Trapping of bead and attachment to gliding cell
DOI: 10.21769/BioProtoc.v395 Published: Feb 5, 2017 Views: 5061
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(step C11). Focused point of laser beam is indicated by a red circle, marked ‘Trap center’. At 5 sec, a flowing bead came from the right side and trapped at the beam. At 18 sec, a gliding cell was moved into the field by stage handling. At 27 sec, the bead was attached to the gliding cell. The cell pulled the trapped bead and stalled from 33 sec to the end.
Extracted from protocol
Force Measurement on Mycoplasma mobile Gliding Using Optical Tweezers
Mizutani, M. and Miyata, M. (2017). Bio-protocol 7(3): e2127.
DOI: 10.21769/BioProtoc.2127.
(step C11). Focused point of laser beam is indicated by a red circle, marked ‘Trap center’. At 5 sec, a flowing bead came from the right side and trapped at the beam. At 18 sec, a gliding cell was moved into the field by stage handling. At 27 sec, the bead was attached to the gliding cell. The cell pulled the trapped bead and stalled from 33 sec to the end.
Extracted from protocol
Force Measurement on Mycoplasma mobile Gliding Using Optical Tweezers
Mizutani, M. and Miyata, M. (2017). Bio-protocol 7(3): e2127.
DOI: 10.21769/BioProtoc.2127.
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Keywords
Mycoplasma, Optical tweezers, Force, Avidin-biotin, Bead
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