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Transfer of seedlings in the observation chamber.
DOI: 10.21769/BioProtoc.v39 Published: Apr 20, 2017 Views: 6370
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Cut out a piece of ½ MS-agar, add ½ MS and align carefully seedlings prior putting back the squared piece of ½ MS-agar. Proceed to observation (FM4-64 staining) or let it stand several hours in a growth chamber so that seedlings acclimate to the Lab-Tek® chamber.
Extracted from protocol
Automated Tracking of Root for Confocal Time-lapse Imaging of Cellular Processes
Doumane, M., Lionnet, C., Bayle, V., Jaillais, Y. and Caillaud, M. (2017). Bio-protocol 7(8): e2245.
DOI: 10.21769/BioProtoc.2245.
Cut out a piece of ½ MS-agar, add ½ MS and align carefully seedlings prior putting back the squared piece of ½ MS-agar. Proceed to observation (FM4-64 staining) or let it stand several hours in a growth chamber so that seedlings acclimate to the Lab-Tek® chamber.
Extracted from protocol
Automated Tracking of Root for Confocal Time-lapse Imaging of Cellular Processes
Doumane, M., Lionnet, C., Bayle, V., Jaillais, Y. and Caillaud, M. (2017). Bio-protocol 7(8): e2245.
DOI: 10.21769/BioProtoc.2245.
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Keywords
Cell division, Mitosis, Cytokinesis, Root, Microscopy, Tracking, Arabidopsis, Phosphoinositide
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