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Preparation of the Lab-Tek® observation chamber for FM4-64 staining.
DOI: 10.21769/BioProtoc.v38 Published: Apr 20, 2017 Views: 6358
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Pour 3 ml of ½ MS-0.8% agar with 5 μl of 100 mg/ml FM4-64 in the Lab-Tek® observation chamber.
Extracted from protocol
Automated Tracking of Root for Confocal Time-lapse Imaging of Cellular Processes
Doumane, M., Lionnet, C., Bayle, V., Jaillais, Y. and Caillaud, M. (2017). Bio-protocol 7(8): e2245.
DOI: 10.21769/BioProtoc.2245.
Pour 3 ml of ½ MS-0.8% agar with 5 μl of 100 mg/ml FM4-64 in the Lab-Tek® observation chamber.
Extracted from protocol
Automated Tracking of Root for Confocal Time-lapse Imaging of Cellular Processes
Doumane, M., Lionnet, C., Bayle, V., Jaillais, Y. and Caillaud, M. (2017). Bio-protocol 7(8): e2245.
DOI: 10.21769/BioProtoc.2245.
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Keywords
Cell division, Mitosis, Cytokinesis, Root, Microscopy, Tracking, Arabidopsis, Phosphoinositide
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