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The 3D confocal imaging of S-phase marker, EdU, within a cleared adult zebrafish brain.
DOI: 10.21769/BioProtoc.v362 Published: Aug 5, 2019 Views: 2493
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Following a 2 h EdU (green) pulse (Dimensions: 2,000 μm x 3,600 μm x 1,100 μm). Note: Green autofluorescence in the vasculature, especially prominent in the optic tectum and cerebellum.
Extracted from protocol
Looking through Brains with Fast Passive CLARITY: Zebrafish, Rodents, Non-human Primates and Humans
Mortazavi, F., Stankiewicz, A. J. and Zhdanova, I. V. (2019). Bio-protocol 9(15): e3321.
DOI: 10.21769/BioProtoc.3321.
Following a 2 h EdU (green) pulse (Dimensions: 2,000 μm x 3,600 μm x 1,100 μm). Note: Green autofluorescence in the vasculature, especially prominent in the optic tectum and cerebellum.
Extracted from protocol
Looking through Brains with Fast Passive CLARITY: Zebrafish, Rodents, Non-human Primates and Humans
Mortazavi, F., Stankiewicz, A. J. and Zhdanova, I. V. (2019). Bio-protocol 9(15): e3321.
DOI: 10.21769/BioProtoc.3321.
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Keywords
CLARITY, Immunohistochemistry, Brain, Imaging, Whole-tissue staining, Adult neurogenesis, Zebrafish, Rat, Mice, Rhesus monkey, Non-human primate, Human
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