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Capsid uncoating assay via painting with CypA.
DOI: 10.21769/BioProtoc.v361 Published: Jul 5, 2019 Views: 2014
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Image stack of the GFP channel (content marker), AF568 channel (CypA paint), and merge of both videos of a typical capsid uncoating assay via painting with CypA (80 x 60 pixel region of the field of view). Images were acquired at 1 frame per second.
Extracted from protocol
Fluorescence Microscopy Assay to Measure HIV-1 Capsid Uncoating Kinetics in vitro
Márquez, C. L., Lau, D., Walsh, J., Rifat Faysal, K., Parker, M. W., Turville, S. G. and Böcking, T. (2019). Bio-protocol 9(13): e3297.
DOI: 10.21769/BioProtoc.3297.
Image stack of the GFP channel (content marker), AF568 channel (CypA paint), and merge of both videos of a typical capsid uncoating assay via painting with CypA (80 x 60 pixel region of the field of view). Images were acquired at 1 frame per second.
Extracted from protocol
Fluorescence Microscopy Assay to Measure HIV-1 Capsid Uncoating Kinetics in vitro
Márquez, C. L., Lau, D., Walsh, J., Rifat Faysal, K., Parker, M. W., Turville, S. G. and Böcking, T. (2019). Bio-protocol 9(13): e3297.
DOI: 10.21769/BioProtoc.3297.
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Keywords
Retrovirus, HIV, Capsid stability assay, Uncoating, CypA, Viral particles, CA
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