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BL-CFC culture following over-expression of eight key transcription factors.
DOI: 10.21769/BioProtoc.v357 Published: Jan 5, 2019 Views: 2666
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The video is a 48-h-time-lapse microscopy analysis of BL-CFC culture in presence of doxycycline, i.e., following the over-expression of eight key transcription factors (Runx1, Cbfb, Gata2, Tal1, Fli1, Lyl1, Erg and Lmo2).
Extracted from protocol
Quantification of Mouse Hematopoietic Progenitors’ Formation Using Time-lapse Microscopy and Image Analysis
Bergiers, I., Tischer, C., Bölükbaşı, Ö. V. and Lancrin, C. (2019). Bio-protocol 9(1): e3137.
DOI: 10.21769/BioProtoc.3137.
The video is a 48-h-time-lapse microscopy analysis of BL-CFC culture in presence of doxycycline, i.e., following the over-expression of eight key transcription factors (Runx1, Cbfb, Gata2, Tal1, Fli1, Lyl1, Erg and Lmo2).
Extracted from protocol
Quantification of Mouse Hematopoietic Progenitors’ Formation Using Time-lapse Microscopy and Image Analysis
Bergiers, I., Tischer, C., Bölükbaşı, Ö. V. and Lancrin, C. (2019). Bio-protocol 9(1): e3137.
DOI: 10.21769/BioProtoc.3137.
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Keywords
Hematopoietic progenitors, Embryonic stem cells, Differentiation, Inducible cell line, Hemangioblast culture, Microscopy, Time-lapse imaging, Round cell counting
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