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GFP::RAD-51 in spo-11 background.
DOI: 10.21769/BioProtoc.v355 Published: Dec 20, 2018 Views: 2271
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Example output of the gfp::rad-51; spo-11 strain before (first frame) and after microirradiation (all following frames). Green = GFP::RAD-51. A sample analysis of this same movie is presented in Figure 5.
Extracted from protocol
Microirradiation for Precise, Double-strand Break Induction in vivo in Caenorhabditis elegans
Harrell, K. E., Koury, E. and Smolikove, S. (2018). Bio-protocol 8(24): e3130.
DOI: 10.21769/BioProtoc.3130.
Example output of the gfp::rad-51; spo-11 strain before (first frame) and after microirradiation (all following frames). Green = GFP::RAD-51. A sample analysis of this same movie is presented in Figure 5.
Extracted from protocol
Microirradiation for Precise, Double-strand Break Induction in vivo in Caenorhabditis elegans
Harrell, K. E., Koury, E. and Smolikove, S. (2018). Bio-protocol 8(24): e3130.
DOI: 10.21769/BioProtoc.3130.
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Keywords
Microirradiation, Double-strand breaks, Protein recruitment, DSB repair, Live imaging, C. elegans
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