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Translation movie of a juvenile zebrafish brain after tissue clearing, anti-GFP and DiI staining along the optical axis: each slice of the confocal stack was converted into a frame of this movie.
DOI: 10.21769/BioProtoc.v354 Published: Dec 5, 2017 Views: 6283
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Video legend
Translation direction from ventral to dorsal; caudal to the right. The bounding box dimensions of the underlying stack are 3.2 x 2 x 1.5 mm, the isotropic voxel size is 1.74 µm. Original bit depth, 12 bits. Green: ETvmat2: GFP expression pattern; Magenta: DiI fiducial staining.
Extracted from protocol
zPACT: Tissue Clearing and Immunohistochemistry on Juvenile Zebrafish Brain
Affaticati, P., Simion, M., De Job, E., Rivière, L., Hermel, J., Machado, E., Joly, J. and Jenett, A. (2017). Bio-protocol 7(23): e2636.
DOI: 10.21769/BioProtoc.2636.
Translation direction from ventral to dorsal; caudal to the right. The bounding box dimensions of the underlying stack are 3.2 x 2 x 1.5 mm, the isotropic voxel size is 1.74 µm. Original bit depth, 12 bits. Green: ETvmat2: GFP expression pattern; Magenta: DiI fiducial staining.
Extracted from protocol
zPACT: Tissue Clearing and Immunohistochemistry on Juvenile Zebrafish Brain
Affaticati, P., Simion, M., De Job, E., Rivière, L., Hermel, J., Machado, E., Joly, J. and Jenett, A. (2017). Bio-protocol 7(23): e2636.
DOI: 10.21769/BioProtoc.2636.
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Keywords
PACT, Tissue clearing, Deep imaging, Zebrafish, Immunohistochemistry, Confocal microscopy
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