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Peroxisome movement in fibroblasts after loss of ACBD5.
DOI: 10.21769/BioProtoc.v351 Published: Sep 5, 2017 Views: 4386
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Video legend
Video 2 shows increased movement of peroxisomes in human skin fibroblasts silenced for ACBD5. Peroxisomes were labelled as described (Video 1). Note that loss of ACBD5, which tethers peroxisomes to the ER, results in increased peroxisomal movement. Not all of the movements may depend on microtubules. Bar = 20 µm. Video 2 (2 min observation, 10 x original speed) is taken from Costello et al. (2017b).
Extracted from protocol
Peroxisome Motility Measurement and Quantification Assay
Metz, J., Castro, I. G. and Schrader, M. (2017). Bio-protocol 7(17): e2536.
DOI: 10.21769/BioProtoc.2536.
Video 2 shows increased movement of peroxisomes in human skin fibroblasts silenced for ACBD5. Peroxisomes were labelled as described (Video 1). Note that loss of ACBD5, which tethers peroxisomes to the ER, results in increased peroxisomal movement. Not all of the movements may depend on microtubules. Bar = 20 µm. Video 2 (2 min observation, 10 x original speed) is taken from Costello et al. (2017b).
Extracted from protocol
Peroxisome Motility Measurement and Quantification Assay
Metz, J., Castro, I. G. and Schrader, M. (2017). Bio-protocol 7(17): e2536.
DOI: 10.21769/BioProtoc.2536.
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Keywords
Peroxisome motility, Live-cell imaging, Organelle cooperation, Membrane contact, GFP-PTS1, ACBD5, ACBD4
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