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Peroxisome movement in control fibroblasts.
DOI: 10.21769/BioProtoc.v350 Published: Sep 5, 2017 Views: 4396
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Video legend
Video 1 shows movement of peroxisomes in human skin fibroblasts (control) transfected with the peroxisome marker EGFP-SKL (fluorescent reporter with a C-terminal peroxisomal targeting signal). Note the spherical, punctate labelling of peroxisomes and some long-range, directed movements, which are likely microtubule-dependent. Bar = 20 µm. Video 1 (2 min observation, 10 x original speed) is taken from Costello et al. (2017b).
Extracted from protocol
Peroxisome Motility Measurement and Quantification Assay
Metz, J., Castro, I. G. and Schrader, M. (2017). Bio-protocol 7(17): e2536.
DOI: 10.21769/BioProtoc.2536.
Video 1 shows movement of peroxisomes in human skin fibroblasts (control) transfected with the peroxisome marker EGFP-SKL (fluorescent reporter with a C-terminal peroxisomal targeting signal). Note the spherical, punctate labelling of peroxisomes and some long-range, directed movements, which are likely microtubule-dependent. Bar = 20 µm. Video 1 (2 min observation, 10 x original speed) is taken from Costello et al. (2017b).
Extracted from protocol
Peroxisome Motility Measurement and Quantification Assay
Metz, J., Castro, I. G. and Schrader, M. (2017). Bio-protocol 7(17): e2536.
DOI: 10.21769/BioProtoc.2536.
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Keywords
Peroxisome motility, Live-cell imaging, Organelle cooperation, Membrane contact, GFP-PTS1, ACBD5, ACBD4
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