Filling procedure of reference/sample cell.

S Saponaro, A.

DOI: 10.21769/BioProtoc.v341 Published: Aug 5, 2018 Views: 12930

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The video shows the steps necessary for the proper loading of the solutions into the reference/sample cells (see Steps 5a-5c). For simplicity, the video describes only the filling of the sample cell.

Extracted from protocol
Isothermal Titration Calorimetry: A Biophysical Method to Characterize the Interaction between Label-free Biomolecules in Solution
Saponaro, A. (2018). Bio-protocol 8(15): e2957.
DOI: 10.21769/BioProtoc.2957.

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Keywords

ITC, Calorimetr, Macromolecular interaction, Binding affinity, KAT1 channels, 14-3-3 proteins

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