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Tilt series of type I PD.
DOI: 10.21769/BioProtoc.v339 Published: Jan 5, 2018 Views: 4966
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Full range tilt series (-65° to +65°) of the type I plasmodesma (in the centre) found in 4 days old cultured cells, processed with this protocol and shown in Figure 5. Dense black spots are the fiducial markers used for alignment with Etomo.
Extracted from protocol
Electron Tomography to Study the Three-dimensional Structure of Plasmodesmata in Plant Tissues–from High Pressure Freezing Preparation to Ultrathin Section Collection
Nicolas, W. J., Bayer, E. and Brocard, L. (2018). Bio-protocol 8(1): e2681.
DOI: 10.21769/BioProtoc.2681.
Full range tilt series (-65° to +65°) of the type I plasmodesma (in the centre) found in 4 days old cultured cells, processed with this protocol and shown in Figure 5. Dense black spots are the fiducial markers used for alignment with Etomo.
Extracted from protocol
Electron Tomography to Study the Three-dimensional Structure of Plasmodesmata in Plant Tissues–from High Pressure Freezing Preparation to Ultrathin Section Collection
Nicolas, W. J., Bayer, E. and Brocard, L. (2018). Bio-protocol 8(1): e2681.
DOI: 10.21769/BioProtoc.2681.
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Keywords
Plasmodesmata, Plant, Cell wall, Electron tomography, Electron microscopy, Cryofixation
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