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Depletion efficiency in the retina.
DOI: 10.21769/BioProtoc.v331 Published: Aug 20, 2018 Views: 4640
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This video shows a z-scan performed on a confocal laser scanning microscope through representative retinae, which were either treated with the CSF1R-inhibitor PLX5622 (right panel) or control chow (left panel) for 21 d. Microglia (Iba1, red) are only detectable in the control retina. There, they are evenly distributed throughout the retina and mainly localized in the ganglion cell layer (GCL) as well as inner (IPL) and outer plexiform layer (OPL). PLX5622-treated retinae show no microglia in any layer. Staining with the neuronal marker βIII-tubulin (green) shows retinal ganglion cells in the GCL and binding of the secondary antibody to IgG in the non-perfused retina shows positively stained blood vessels, thereby indicating the IPL and OPL.
Extracted from protocol
Studying the Role of Microglia in Neurodegeneration and Axonal Regeneration in the Murine Visual System
Hilla, A. M. and Fischer, D. (2018). Bio-protocol 8(16): e2979.
DOI: 10.21769/BioProtoc.2979.
This video shows a z-scan performed on a confocal laser scanning microscope through representative retinae, which were either treated with the CSF1R-inhibitor PLX5622 (right panel) or control chow (left panel) for 21 d. Microglia (Iba1, red) are only detectable in the control retina. There, they are evenly distributed throughout the retina and mainly localized in the ganglion cell layer (GCL) as well as inner (IPL) and outer plexiform layer (OPL). PLX5622-treated retinae show no microglia in any layer. Staining with the neuronal marker βIII-tubulin (green) shows retinal ganglion cells in the GCL and binding of the secondary antibody to IgG in the non-perfused retina shows positively stained blood vessels, thereby indicating the IPL and OPL.
Extracted from protocol
Studying the Role of Microglia in Neurodegeneration and Axonal Regeneration in the Murine Visual System
Hilla, A. M. and Fischer, D. (2018). Bio-protocol 8(16): e2979.
DOI: 10.21769/BioProtoc.2979.
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Keywords
Axon regeneration, Neurodegeneration, Microglia, Microglia depletion, PLX5622, CSF1R
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