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Resuspending pellet in buffer I or buffer II: Gently rotate bottles in an ice-bucket to resuspend the bacterial pellet in buffer
DOI: 10.21769/BioProtoc.v319 Published: Nov 5, 2017 Views: 12757
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Resuspending pellet in buffer I or buffer II: Gently rotate bottles in an ice-bucket to resuspend the bacterial pellet in buffer
Extracted from protocol
A Quick and Easy Method for Making Competent Escherichia coli Cells for Transformation Using Rubidium Chloride
Sharma, N., Anleu Gil, M. X. and Wengier, D. (2017). Bio-101: e2590.
DOI: 10.21769/BioProtoc.2590.
Resuspending pellet in buffer I or buffer II: Gently rotate bottles in an ice-bucket to resuspend the bacterial pellet in buffer
Extracted from protocol
A Quick and Easy Method for Making Competent Escherichia coli Cells for Transformation Using Rubidium Chloride
Sharma, N., Anleu Gil, M. X. and Wengier, D. (2017). Bio-101: e2590.
DOI: 10.21769/BioProtoc.2590.
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Keywords
Competent cells, E. coli, Transformation efficiency, TOP10, DH5α
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